Composite

Part:BBa_K1824003:Design

Designed by: Wenbo Xu, Hao Wu   Group: iGEM15_XJTLU-CHINA   (2015-09-05)


pBAD + RNA Thermometer A1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequence


Source

pBAD was from the Registry, and A1 was from BIT-CHINA.


References